[phenixbb] Improvement of low resolution MR phases
John R Helliwell
jrhelliwell at gmail.com
Tue Jun 29 01:13:55 PDT 2021
I have thought before about this kind of case, of 5 to 6 Angstrom resolution diffraction, namely that the molecular transform must increase again at higher resolution, say 2 1/2 Angstrom, at which therefore the measurable intensity would increase enough to maybe be above background.
To check this go back to your diffraction images and integrate to, say, 2Angstrom. Does the <I/sigI> show the increase at around 2 1/2 Angstrom resolution?
Obviously if you do have those higher resolution data measurable then matters improve all round.
Emeritus Professor John R Helliwell DSc
> On 28 Jun 2021, at 12:56, Kajander, Tommi A <tommi.kajander at helsinki.fi> wrote:
> Hello all,
> I was wondering what would be current best protocols for trying to improve maps/phases for low resolution
> MR / refinement solutions (5-6 Å resolution).
> High solvent content of course, dimeric 2:2 protein complex so some NCS averaging and density modification
> I assume might help (related structures known, currently maps very blobby, clear solution but I think should be able to do better...).
> Also, refinement suggestions welcome.
> Best route in phenix? So far worked initially with ccp4. Other suggestions also welcome.
> Tommi Kajander, Ph.D.
> Institute of Biotechnology
> University of Helsinki
> Viikinkaari 1 (P.O. Box 65)
> 00014 Helsinki, Finland
> p. +358-50-4480991
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