[phenixbb] Overall map resolution (d_FSC) x local map resolution

Christopher Schlicksup cjschlicksup at lbl.gov
Mon Aug 9 07:45:20 PDT 2021

Hi Carlos, differences like this do not seem abnormal. For local
resolution, it is common to get a very broad range of values (ie, 2.8-10Å),
but usually the most frequently occurring value is close to the value
provided with full-map methods. It can be tough to visualize with color
sometimes, but if you look at the volume histogram of the local resolution
map in Chimera you can see the distribution of resolution values.

For subsequent Phenix programs and for data reporting, I suggest using the
full map value.

On Mon, Aug 9, 2021 at 1:59 AM Carlos HENRIQUE FERNANDES <
carlos.henrique_fernandes at sorbonne-universite.fr> wrote:

> Dear all,
> I've been finding some discordance between overall map resolution value
> obtained at phenix.mtriage (d_FSC) and local map resolution values obtained
> at phenix.local_resolution.
> phenix.mtriage (using full map and both half maps) provides a d_FSC of
> 4.31 Å; however, the phenix.local_resolution (using both half maps and
> not informing the d_FSC) provides local map resolution values have a range
> of 3.8 - 4.4 Å with most of the map having 4.0 Å resolution with very few
> parts (only small flexible regions at terminal regions) having 4.3-4.4 Å
> resolution.
> Are these differences normal or is there something wrong with my map
> refinement? Despite d_FSC being 4.31 Å; can I consider my map at 4.0 Å
> resolution and do the sharpening and dock in map operations at 4.0 Å?
> Thanks in advance,
> Carlos Fernandes
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