[phenixbb] d99 beyond Nyquist?

Ricardo Righetto ricardorighetto at gmail.com
Fri Jun 1 10:20:55 PDT 2018


I've seen the d99 criterion in phenix.mtriage reporting a resolution value
beyond Nyquist (=2.0 A), or very close to Nyquist in another case. For
these particular maps, which are from the same dataset processed with two
different single-particle analysis packages (internal benchmarking),
half-map FSC-based methods (including blocres) report resolutions in the
range of 3.0-3.5 A which are consistent with the features I can see.

How can I interpret this? I understand that d99 starts omitting Fourier
samples from the corners of the reciprocal box, but does it make sense to
report a value beyond Nyquist?
I tried supplying unfiltered, masked and unmasked versions of the maps with
barely any variation in the d99 results.

Thanks in advance for helping figuring this out!

Best wishes,

Ricardo Diogo Righetto
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