[phenixbb] Gold atoms in cryo-EM

a.malay at riken.jp a.malay at riken.jp
Sun Sep 10 22:23:48 PDT 2017

Dear all,

I hope that this question is neither too off-topic or naive.

I am trying to refine a large protein structure against a cryo-EM map using Phenix real-space refinement. The protein complex is unusual in that the oligomeric state is held together via coordinated gold atoms. However, there seems to be a problem with refinement, in that the the gold atoms show extremely high B-factors (>200) after refinement, whereas the B-factors of the protein residues, including those surrounding the gold atoms, adopt more reasonable values.

My question is: could the unusually high B-factors of the gold atoms be somehow due to differences in the relative scattering of Au atoms when obtained from X-ray vs. electron beam irradiation? And if so, is there any way to correct for this? 

Thank you in advance for your kind help.


More information about the phenixbb mailing list