[phenixbb] [ccp4bb] Modify methionine to acetylated methionine in coot

[Anshul Bhardwaj]

Thanks Paul for the quick response. Simple enough and works beautifully for modifying N-terminus methionine to AME in coot.
Anshul Bhardwaj

________________________________________
From: Paul Emsley <pemsley at mrc-lmb.cam.ac.uk>
Sent: Monday, January 25, 2016 7:39 PM
To: Anshul Bhardwaj; COOT at jiscmail.ac.uk
Subject: Re: [ccp4bb] Modify methionine to acetylated methionine in coot

On 25/01/2016 23:33, Anshul Bhardwaj wrote:
> Hello,

Hello.
> How to replace 'M' with 'AME' in coot itself? an option like mutate residue, etc.
> I couldn't figure out right away so I worked my way around by simply copying AME from one of the pdb from RCSB database and replaced methionine in my model. Linked AME to residue# 2 in coot and refinement was straightforward after generating restraints for the modified model. So it worked for now but still curious as there has to be a way to replace 'M' with 'AME' in coot, isn't it (?).
>

Generally, for this sort of thing one would use:

Extensions -> Modelling -> Replace Residue -> AME

But this might not work in the case because the modification is on the
main-chain not the side-chain.  But it might do if the AME is at the
N-terminus.

This thread might shed some light on things:

https://www.mail-archive.com/coot@jiscmail.ac.uk/msg02536.html

Paul.

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