[phenixbb] Sulfur SAD

Ivanov ivanov at embl.fr
Mon Jul 27 11:02:08 PDT 2015

Hi Mohamed,

I solved a structure at 1.5 A with Sulfur MAD. There were only 4 Sulfurs in 
the sequence and the signal has very nice.

Pay attention S does not give high anomalous signal.

I suggest you collect a dataset at 7kev (around 2.0A) which is the 
absorption edge of S. You must collect very high multiplicity around 30-40 
times with low intensity beam not to burn the crystal. This at kappa 0 and 
kappa 45. Then you could merge both datasets. I remember an important 
thing, on the absorption edge we had to be careful on the harmonics and 
make a nice setting on the beam to remove them. I suggest you do also a 
collection at 14kev far away from absorption edge.

For my case only Autorickshaw found the Sulfurs and solve the problem of 

Hope it helps,

Sent with AquaMail for Android

On 27 July 2015 7:17:18 pm mohamed noor <mohamed.noor34 at gmail.com> wrote:

> Dear all
> Would you have some tips on collecting datasets for S-SAD at a 'normal'
> beamline (tunable from 0.5-2.5 A)? How helpful/important is it to use mini
> kappa?
> Thanks.
> ----------
> _______________________________________________
> phenixbb mailing list
> phenixbb at phenix-online.org
> http://phenix-online.org/mailman/listinfo/phenixbb
> Unsubscribe: phenixbb-leave at phenix-online.org
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20150727/4023387f/attachment.htm>

More information about the phenixbb mailing list