[phenixbb] on determination whether a protein is a monomer or complex by phenix

Jobichen Chacko jobichenc at gmail.com
Mon Jul 6 22:54:54 PDT 2015

Dear Smith,
You can try Phenix-xtriage which will give you the number of residues
possible in the asymmetric unit with 50% solvent content. But the
solvent content can vary between 28% to 80% and accordingly the number
of molecules/residues in the asymmetric unit can vary. The Matthews
probability calculator (online tool as well in ccp4i) will also give
you an idea of the number of molecules with different solvent content.

If you are looking for number of copies of a protein-complex or
protein-monomer, these results are inconclusive. Need to solve the
structure to get a definite answer.


On Mon, Jul 6, 2015 at 5:20 PM, Smith Liu <smith_liu123 at 163.com> wrote:
> Dear All,
> Suppose I have got a mtz file, and I know the aa sequence of the protein
> from which I got the crystal and the corresponding mtz file, will you please
> tell me which phenix function (program) can tell me whether my protein
> crystal was formed by protein monomer or by protein complex, and if formed
> by protein complex, how many subunits existing in the complex?
> Best regards.
> Smith
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