[phenixbb] Phasing using CryoEM Map

Pavel Afonine pafonine at lbl.gov
Mon Sep 1 22:32:57 PDT 2014

Hi Alex,

> I trying to phase my 4Å data with low resolution cryoEM maps (various 
> 12-18Å). There is some documentation, but not really for dealing with 
> EM data. I am having trouble converting the EM data from mrc to 
> suitable F's for phaser.

isn't it a matter of Fourier transforming your map into structure 
factors and then using them down the road pretending that these are your 
"Fobs" and however obtained phases?

If that's the case then phenix.map_to_structure_factors is the tool you 

If not I wonder what am I missing?


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