[phenixbb] Phasing using CryoEM Map

Terwilliger, Thomas Charles terwilliger at lanl.gov
Sat Aug 30 12:19:18 PDT 2014

Hi Alex,

On the TaBr cluster:  I'm sorry as you noticed this was broken in hyss and autosol.  It was just fixed a week ago...however we have not had any builds released since then. Once the next nightly build is released you should be able to use "TX" as your atom type in either hyss or autosol.

All the best,
Tom T

From: phenixbb-bounces at phenix-online.org [phenixbb-bounces at phenix-online.org] on behalf of Alexander Kintzer [afkintzer at gmail.com]
Sent: Saturday, August 30, 2014 12:08 PM
To: phenixbb at phenix-online.org
Subject: [phenixbb] Phasing using CryoEM Map

Hello All,

I trying to phase my 4Å data with low resolution cryoEM maps (various 12-18Å). There is some documentation, but not really for dealing with EM data. I am having trouble converting the EM data from mrc to suitable F's for phaser.
One would want to try different density contours of the cryoEM map, but I haven't found a good way to accomplish this.

I also have been unable to search for Ta6Br12 clusters using Autosol even though the documentation seems to suggest that it is possible using the "TX" or "XX" designation for heavy atom search.

Any guidance would be extremely helpful.

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