[phenixbb] mr_rosetta

Terwilliger, Thomas Charles terwilliger at lanl.gov
Wed Aug 20 10:34:34 PDT 2014

Hi Felipe,

You can try adding the second model in as a fixed model:

Fixed_model = None A fixed model can be added. This will be added as a fixed model in molecular replacement. The sequence for this model should be included in your sequence file.

If that doesn't work, let me know!
All the best,
Tom T

From: phenixbb-bounces at phenix-online.org [phenixbb-bounces at phenix-online.org] on behalf of Felipe Trajtenberg [felipet at pasteur.edu.uy]
Sent: Wednesday, August 20, 2014 11:02 AM
To: phenixbb at phenix-online.org
Subject: [phenixbb] mr_rosetta

Dear all

I am trying to perform MR of a multidomain protein using
phenix.mr_rosetta and building each domain sequentially. The first
search works perfect and I have a potential solution. In the second
search I am having some troubles in setting up the process. When I fixed
the best first model and look for a second domain I found that rosetta
only rebuilds the chain A, which was the one that already was fixed and
chain B disappears.

how can I fix one domain and build the second without loosing any

thanks in advance

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