[phenixbb] few questions about resolving new structure through MR

Zhihong Yu nkyuzhih at gmail.com
Thu Nov 7 10:44:17 PST 2013

Dear  Nathaniel,

Thanks for your reply. So from your answer, I feel that maybe it's not
worth to spend much time to resolve the structure through MR based on
current dataset? Even I can correctly place the domainB, it's still hard to
resolve the intact protein structure without other phasing information, is
that right?

Thanks again and best regards!


> I confess I haven't had to solve a structure like this myself - just seen
> a bunch of datasets where similar attempts didn't work - but it's hard to
> see how it can be solved without experimental phasing information (SeMet,
> etc.).  The good news is that if you can get a correct MR solution for
> domainB, you can use MR-SAD to find heavy atom sites and solve the
> structure, which should be easier than starting from no phases at all.
> -Nat
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