[phenixbb] Low resolution structure

Kendall Nettles knettles at scripps.edu
Thu Mar 11 12:40:19 PST 2010

I have a few questions related to a 3.5 angstrom structure. I have one helix that I am having trouble placing accurately. It does have a cysteine that disulfide bonds with the same residue in a symmetry related molecule. Can I restrain this distance during a rigid body refinement of the helix? How do I do this? What kind of parameters would be appropriate for the disulfide?

My second question is whether someone can suggest appropriate distance_ideal,    sigma, and  slack for hydrogen bond restraints for a helix? Also, I have a 2 angstrom structure of this part of the complex for reference.

Kendall Nettles
-------------- next part --------------
An HTML attachment was scrubbed...
URL: <http://phenix-online.org/pipermail/phenixbb/attachments/20100311/4b6d6ce8/attachment-0003.htm>

More information about the phenixbb mailing list