[phenixbb] NAG-NAG description

Chris Ulens chris.ulens at med.kuleuven.be
Wed Jun 2 12:27:21 PDT 2010

As a follow-up on the NAG-NAG question I was wondering if other insect  
cell users could point me to the proper sources in the literature that  
describe the most common glycosylation forms in Sf9/Sf21 insect cells.  
It seems we have chains composed of up to 4 sugar molecules attached  
to an Asn residue and it would be helpful to know which sugars are  
most likely to follow after NAG-NAG.


On Jun 1, 2010, at 1:44 PM, Chris Ulens wrote:

Thanks for the feedback.
I would like to refine a NAG-NAG chain attached to residue Asn122 in
my structure. There are 5 identical subunits.
Could you verify my .params file?
I get the following error:
Empty atom selection
.pdb_interpretation.apply_cif_link.residue_selection_1="chain A and
resname NAG and resid 122"



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