[phenixbb] phenixbb Digest, Vol 56, Issue 30

Vellieux Frederic Frederic.Vellieux at ibs.fr
Thu Jul 29 00:23:56 PDT 2010

What about trying heavy atom phasing? Like introducing Se-Mets in your 
protein for MAD data collection. Sometimes you can waste plenty of time 
trying molecular replacement and the solution never comes out. 
particularly when you have no sequence homology...


shuchi moni wrote:
>         i have one data of 3 A of a unknown protein don't have
>         sequence homlogous pdb known . but showing highly coserved fold. 
> space group is p622 and showing hexamer in assymmetric unit . in 
> molecuar replacement with a single monomer when i m searching 6 copies 
> lots of clashes are coming. with single copy serach i m getting fully 
> fit template in density but unable to search density for others 
> ensembles.  how should i proceed for this
> regards
> vandana kukshal
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