[phenixbb] questions about ncs copy in autobuild

Randy J. Read rjr27 at cam.ac.uk
Fri Jul 23 11:07:06 PDT 2010

Something we've been using recently in our own work is to average the 
density, cut it out, and then use the density as a model to look for 
additional copies. This works pretty well, so we will be making it easier 
to do within Phenix, but at the moment it's not trivial.

I'm just en route to a holiday, so I won't be able to help for a couple of 
weeks, but if you'd like some help trying that option, get in touch with me 
(preferably off-list) in the second week of August.

All the best,


On Jul 22 2010, vandana wrote:

>i have one 3.27 A data of one of my protein. it is a multidomain protein .
>i  used molecular repacement (phaser ) to find individual domains i got
>density for 3 domains.
>with last 4th domain i am getting solution with 5 clashes and density is
>nort clear for that . so i used phenix find helix and strands module i got
>43 fragments model . now i dont know how to proceed further how i can start
>fitting. suggest me for further step .

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