[phenixbb] Phaser and anisotropy question

Pavel Afonine PAfonine at lbl.gov
Thu Sep 10 10:19:31 PDT 2009

Hi Peter,

> Will this improved anisotropic scaling be implemented in other modules 
> of phenix.refine ? If not do you advise using F_ISO, SIGF_ISO from the 
> newer version of phaser as the input labels for refinement  ?

1) phenix.refine does anisotropic correction, see this paper for more 

Acta Cryst. (2005). D61, 850-855. "A robust bulk-solvent correction and 
anisotropic scaling procedure". P. V. Afonine, R. W. Grosse-Kunstleve 
and P. D. Adams.

and slide #20 here:

2) Improved version of anisotropic scaling suggested by R. Read (more 
precisely, a better way of map calculation given high anisotropy) will 
be implemented in future. We discussed it but I didn't get a chance yet 
to implement it.

3) I do not believe that it is a good idea to use manipulated Fobs in 


More information about the phenixbb mailing list