[phenixbb] Best first map for RESOLVE after MR

Peter Grey petgxray at gmail.com
Sun Aug 30 14:32:23 PDT 2009

Hi Pavel,

Thank you very much for your fast answer. I just thought that maybe the bulk
solvent correction procedure in phenix can really create better maps because
with such big complexes I must use all low resolution data. Of course I can
try both options but there are so many things to try with this big complex
at low resolution that I would like to eliminate options where possible.

Many many thanks,


On Sun, Aug 30, 2009 at 7:22 PM, Pavel Afonine <PAfonine at lbl.gov> wrote:

> Hi Peter,
> I guess this my reply will not replace Tom's or Randy's one...
> > Should it be the 2mFo-DFc map directly from Phaser or would it be
> > better to do rigid body refinement in phenix.refine and use the bulk
> > solvent corrected 2mFo-DFc map ?
> - why don't you try using both options and choose the best outcome?
> - running a round of rigid body refinement in phenix.refine and using
> its 2mFo-DFc map will not hurt at least or may make your model and
> therefore the map better, so you will have a better starting point.
> Pavel.
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