[phenixbb] phenix.xtriage says that entire res range contains anomalous signal but chart says differently?
Edward A. Berry
BerryE at upstate.edu
Tue Aug 11 12:30:48 PDT 2009
Sorry, I wasn't thinking. Selenium edge, of course.
No need to backsoak, and
Not likely all three snm would be disordered.
sbiswas2 at ncsu.edu wrote:
> Is there a chance of backsoaking even when the protein has been expressed
> as a selenomethionine derivative? I just overexpressed the protein by
> shutting down the methionine biosynthesis in E.coli and incorporated SeMet
> instead of methionine.
> The protein has three methionine and is 26kDa
>> Did you "backsoak" the crystal? Wouldn't heavy atom in the
>> mother liquor give a fluorescence peak even if none of it bound?
>> sbiswas2 at ncsu.edu wrote:
>>> I had similar problems recently, I did a wavelength scan of my crystal
>>> the synchrotron (selenium edge) and collected one dataset at the peak
>>> wavelength.The scan gives me a distinct peak. However when I processed
>>> data and evaluated in xtriage it says that there is no anomolous signal.
>>> am unable to explain this? any advice?
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