[phenixbb] phenix.xtriage says that entire res range contains anomalous signal but chart says differently?

sbiswas2 at ncsu.edu sbiswas2 at ncsu.edu
Tue Aug 11 11:37:31 PDT 2009

Is there a chance of backsoaking even when the protein has been expressed
as a selenomethionine derivative? I just overexpressed the protein by
shutting down the methionine biosynthesis in E.coli and incorporated SeMet
instead of methionine.
The protein has three methionine and is 26kDa

> Did you "backsoak" the crystal? Wouldn't heavy atom in the
> mother liquor give a fluorescence peak even if none of it bound?
> Ed
> sbiswas2 at ncsu.edu wrote:
>> Hi,
>> I had similar problems recently, I did a wavelength scan of my crystal
>> at
>> the synchrotron (selenium edge) and collected one dataset at the peak
>> wavelength.The scan gives me a distinct peak. However when I processed
>> the
>> data and evaluated in xtriage it says that there is no anomolous signal.
>> I
>> am unable to explain this? any advice?
>> Shya
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