[phenixbb] poor phasing
Thomas C. Terwilliger
terwilliger at lanl.gov
Fri Apr 24 06:20:29 PDT 2009
I would suggest running
on your data, and examining in particular its analysis of
2. possible space groups (are your certain that there is a screw axis?)
3. measureability of anomalous signal as a function of resolution
In 1.4-4 you may have to run both p43212 and p41212 (current versions do
this for you).
All the best,
>> Dear members,
>>> I am using phenix1.4-4 version. In autosol, got the score of 10.24 for
>>> iodine soaked data.
>>> FOM:0.27 and only 54 residues ( that too wrongly) were built out of
>>> 129.(CC=0.4), R=0.49 and Rfree=0.574.
>>> Regrading the data quality, Rsym:0.0448, Ranom:0.055, Resln:25.0-2.6A
>>> Spacegroup is P43212, completeness:99.4%, Multiplicity:11.7, anomalos
>>> signal is 1.52 at lowest resoln and higher in high resolution shells.
>>> What might be reason for not able to solve this structure?
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