[phenixbb] native Patterson peak

David Garboczi dgarboczi at niaid.nih.gov
Thu May 1 10:23:23 PDT 2008

Data was integrated with XDS, indexed on all the 
frames.  No other choice but primitive 
orthorhombic, I think.  I was interpreting it as 
two mols along the y-axis were two fold related, 
which made a translation between them and the 
next au along y.


>How certain are you regarding your space group ?
>The native peak at almost 0 -0.5 0 is suspicious. If you used HKL for
>indexing try something else with multiple images separated by e.g. 15
>degrees and see if you can process your dataset in a different space group.
>David Garboczi wrote:
>>We have datasets measured on crystals of a 19 kD protein that are
>>P212121, with cell constants of 66, 75, 136.
>>Matthew's considerations say 3, 4, or 5 copies per au are probable.
>>xtriage finds a native peak at (0.0, -0.5, 0.036) that is 31 in
>>height (origin=100)
>>No self-rotation function peaks in xprep, that I can understand.
>>Is this clearly translational symmetry that is making MR difficult?
>>Hints as to how to go forward are welcome.
>Jürgen Bosch
>University of Washington
>Dept. of Biochemistry, K-426
>1705 NE Pacific Street
>Seattle, WA 98195
>Box 357742
>Phone:	 +1-206-616-4510
>FAX:	 +1-206-685-7002
>Web:     http://faculty.washington.edu/jbosch
>phenixbb mailing list
>phenixbb at phenix-online.org

David N. Garboczi, PhD
Phone: 301-496-4773
Investigator, Structural Biology Section (SBS)
Laboratory of Immunogenetics (LIG)
National Institute of Allergy and Infectious Diseases (NIAID)
National Institutes of Health (NIH)
Twinbrook 2/Room 110
12441 Parklawn Drive
Rockville, Maryland 20852-1742	   
Fax:    301-402-0284
Email: dgarboczi at niaid.nih.gov

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