[phenixbb] Refining with NCS

Francis E Reyes Francis.Reyes at Colorado.EDU
Mon Aug 25 12:37:53 PDT 2008

Thanks for the reply Pavel

However, with regard to the NCS maps issue, which should I be building  
to?Does the 2fofoc that phenix puts out adjusted for ncs and use that?



On Aug 25, 2008, at 11:59 AM, Pavel Afonine wrote:

> Hi Francis,
>> Also if anyone has a paper/protocol for NCS refinement, I'd love to
>> check it out (to figure out which map to fit to).
> Just something to keep in mind... I always prefer to run two  
> refinements
> in parallel with and without using NCS. It's resolution (and
> data-to-parameters ratio) dependent. I've seen a  number of cases  
> where
> using the NCS is very beneficial and similar number of cases where  
> it's
> better to not use NCS (mostly at resolutions ~2-2.5A).
> I would also check manually the NCS groups that are found by
> phenix.refine automatically. I have some cases where automatic  
> detection
> did not yield a good selection.
> Pavel.
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Francis Reyes M.Sc.
215 UCB
University of Colorado at Boulder

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